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A protocol to assess cell cycle and apoptosis in human and mouse pluripotent cells

  • Author(s): Edel, Michael J
  • Menchon, Cristina
  • Vaquero, Jose
  • Izpisua Belmonte, Juan Carlos
  • et al.
Abstract

Abstract Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.

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