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Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.

  • Author(s): Stroud, Hume;
  • Do, Truman;
  • Du, Jiamu;
  • Zhong, Xuehua;
  • Feng, Suhua;
  • Johnson, Lianna;
  • Patel, Dinshaw J;
  • Jacobsen, Steven E
  • et al.

Published Web Location

https://doi.org/10.1038/nsmb.2735
Abstract

DNA methylation occurs in CG and non-CG sequence contexts. Non-CG methylation is abundant in plants and is mediated by CHROMOMETHYLASE (CMT) and DOMAINS REARRANGED METHYLTRANSFERASE (DRM) proteins; however, its roles remain poorly understood. Here we characterize the roles of non-CG methylation in Arabidopsis thaliana. We show that a poorly characterized methyltransferase, CMT2, is a functional methyltransferase in vitro and in vivo. CMT2 preferentially binds histone H3 Lys9 (H3K9) dimethylation and methylates non-CG cytosines that are regulated by H3K9 methylation. We revealed the contributions and redundancies between each non-CG methyltransferase in DNA methylation patterning and in regulating transcription. We also demonstrate extensive dependencies of small-RNA accumulation and H3K9 methylation patterning on non-CG methylation, suggesting self-reinforcing mechanisms between these epigenetic factors. The results suggest that non-CG methylation patterns are critical in shaping the landscapes of histone modification and small noncoding RNA.

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