Skip to main content
Open Access Publications from the University of California


UC San Francisco Previously Published Works bannerUCSF

The structure of the endogenous ESX-3 secretion system.

  • Author(s): Poweleit, Nicole
  • Czudnochowski, Nadine
  • Nakagawa, Rachel
  • Trinidad, Donovan D
  • Murphy, Kenan C
  • Sassetti, Christopher M
  • Rosenberg, Oren S
  • et al.

The ESX (or Type VII) secretion systems are protein export systems in mycobacteria and many Gram-positive bacteria that mediate a broad range of functions including virulence, conjugation, and metabolic regulation. These systems translocate folded dimers of WXG100-superfamily protein substrates across the cytoplasmic membrane. We report the cryo-electron microscopy structure of an ESX-3 system, purified using an epitope tag inserted with recombineering into the chromosome of the model organism Mycobacterium smegmatis. The structure reveals a stacked architecture that extends above and below the inner membrane of the bacterium. The ESX-3 protomer complex is assembled from a single copy of the EccB3, EccC3, and EccE3 and two copies of the EccD3 protein. In the structure, the protomers form a stable dimer that is consistent with assembly into a larger oligomer. The ESX-3 structure provides a framework for further study of these important bacterial transporters.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View