Effect of single dose ionizing radiation on the cellular proliferation and apparent radiosensitivity of melanoma.
- Author(s): Yohem, KH;
- Bregman, MD;
- Meyskens, FL
- et al.
An important variable in determining in vitro cell survival is the time interval between treatment and assay. One manifestation of radiation damage is the introduction of a division delay in which the post-radiation division rate is less than the pre-radiation division rate. We have examined the influence of length of incubation on radiosensitivity of murine and human melanoma cells in a soft-agar assay. In general, the longer the incubation period the larger was the median colony diameter within control or experimental groups. Hence apparent radiosensitivity decreased with increased length of incubation.