Isolation and Synthesis of a Bacterially Produced Inhibitor of Rosette Development in Choanoflagellates.
- Author(s): Cantley, Alexandra
- Woznica, Arielle
- Beemelmanns, Christine
- KING, Nicole
- Clardy, Jon
- et al.
Published Web Locationhttps://doi.org/10.1021/jacs.6b01190
The choanoflagellate Salpingoeca rosetta is a microbial marine eukaryote that can switch between unicellular and multicellular states. As one of the closest living relatives of animals, this organism has become a model for understanding how multicellularity evolved in the animal lineage. Previously our laboratories isolated and synthesized a bacterially produced sulfonolipid that induces S. rosetta to form multicellular rosettes. In this study, we report the identification of a bacterially produced inhibitor of rosettes (IOR-1) as well as the total synthesis of this molecule and all of its stereoisomers. Our results confirm the previously noted specificity and potency of rosette-modulating molecules, expand our understanding of the complex chemical ecology between choanoflagellates and rosette-inducing bacteria, and provide a synthetic probe template for conducting further mechanistic studies on the emergence of multicellularity.