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Open Access Publications from the University of California

Optimum primer design to perform targeted genome sequencing

  • Author(s): Sadatmousavi, Parastou
  • et al.

In spite of the numerous advances in the area of sequencing, the actual procedure is still expensive, with the price increasing with portion of the genome to be sequenced and the accuracy required. The Polymerase Chain Reaction (PCR) method has been the way to enrich particular genomic regions of interest for next generation sequencing. However, the challenge is to design a large number of primers that work together to amplify a large number of regions in the multiplex PCR process. This study addresses all different issues that may happen in multiplex PCR process and outlines a procedure to design primers to amplify exon regions of genes, which would increase the accuracy and decrease the cost of sequencing

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