RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP
- Author(s): Kellenberger, CA
- Wilson, SC
- Sales-Lee, J
- Hammond, MC
- et al.
Published Web Locationhttps://www.ncbi.nlm.nih.gov/pubmed/?term=RNA-based+fluorescent+biosensors+for+live+cell+imaging+of+second+messengers+cyclic+di-GMP+and+cyclic+AMP-GMP
Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-I riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV. © 2013 American Chemical Society.
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