UC Irvine

The effects of retinal degeneration on the sensitivity of the retina were studied in the Royal College of Surgeons (RCS) rat by measuring the light reflex of the pupil in response to ganzfeld (full field) flashes. Light reflex thresholds were measured for animals from 32 to 683 days of age, and an age-related decrease in sensitivity of 5.2 log units (maximum) was measured. In contrast, thresholds for non-dystrophic albino controls increased only slightly during a comparable period. RCS rat thresholds increased more for short wavelength light than for long wavelength light. The end result was an altered action spectrum of the light reflex which largely, but not exclusively, reflected cone function. Even in cases of advanced degeneration the light reflex thresholds we measured showed significant input from rods. Pupiliary dark adaptation measured following ganzfeld bleaches (10%) with test stimuli of two different wavelengths revealed two mechanisms; a photopic mechanism (gamma max = 520) determined thresholds early in dark adaptation, but later a scotopic mechanism (gamma max = 500) participated in the light reflex.