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Involvement of claudin-7 in HIV infection of CD4(-) cells.

  • Author(s): Zheng, Junying
  • Xie, Yiming
  • Campbell, Richard
  • Song, Jun
  • Massachi, Samira
  • Razi, Miriam
  • Chiu, Robert
  • Berenson, James
  • Yang, Otto O
  • Chen, Irvin SY
  • Pang, Shen
  • et al.
Abstract

Human immunodeficiency virus (HIV) infection of CD4(-) cells has been demonstrated, and this may be an important mechanism for HIV transmission.We demonstrated that a membrane protein, claudin-7 (CLDN-7), is involved in HIV infection of CD4(-) cells. A significant increase in HIV susceptibility (2- to 100-fold) was demonstrated when CLDN-7 was transfected into a CD4(-) cell line, 293T. In addition, antibodies against CLDN-7 significantly decreased HIV infection of CD4(-) cells. Furthermore, HIV virions expressing CLDN-7 on their envelopes had a much higher infectivity for 293T CD4(-) cells than the parental HIV with no CLDN-7. RT-PCR results demonstrated that CLDN-7 is expressed in both macrophages and stimulated peripheral blood leukocytes, suggesting that most HIV virions generated in infected individuals have CLDN-7 on their envelopes. We also found that CLDN-7 is highly expressed in urogenital and gastrointestinal tissues.Together these results suggest that CLDN-7 may play an important role in HIV infection of CD4(-) cells.

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