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System-level perturbations of cell metabolism using CRISPR/Cas9

  • Author(s): Jakočiūnas, T
  • Jensen, MK
  • Keasling, JD
  • et al.

Published Web Location

https://linkinghub.elsevier.com/retrieve/pii/S0958-1669(16)30208-7
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Abstract

© 2017 Elsevier Ltd CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modifications and transcriptional regulations. The effectiveness of these tools allows researchers to implement genome-wide perturbations, test model-guided genome editing strategies, and perform transcriptional reprogramming perturbations in a more advanced manner than previously possible. In this mini-review we highlight recent studies adopting CRISPR/Cas9 for systems-level perturbations and model-guided metabolic engineering.

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