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ANALYSIS OF TRYPTOPHAN AND ITS METABOLITES BY REVERSE-PHASE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

  • Author(s): TARR, JB;
  • ARDITTI, J
  • et al.
Abstract

Rapid separation and determination of tryptophan, N-formylkynurenine, kynurenine, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, nicotinic acid, nicotinamide, trigonelline, kynurenic acid and xanthurenic acid from pea seedling using reverse-phase high-pressure liquid chromatography is described. Accuracy of the determinations was > 5%, and < 50 pmol of each metabolite could be measured reliably.

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