Skip to main content
eScholarship
Open Access Publications from the University of California

UC San Diego

UC San Diego Electronic Theses and Dissertations bannerUC San Diego

RNAs Regulate TOP1 Protein Interactome

  • Author(s): Orozco, Paola
  • Advisor(s): Lauberth, Shannon M
  • et al.
No data is associated with this publication.
Abstract

Human Topoisomerase 1 (TOP1) is a dynamic enzyme that functions to overcome topological constraints on the DNA that are caused by strand separation. In this study, we demonstrate that TOP1 is an RNA binding protein (RBP) as revealed by ultraviolet-crosslinked RNA immunoprecipitation (UV-RIP) analyses at intergenic and promoter regions in colon cancer cells treated with acute doses of tumor necrosis alpha (TNF-. Through biochemical methods, we found that TOP1 directly interacts with different RNAs. Furthermore, proteomic analyses reveal that RNAs add an additional layer of regulation by contributing to the maintenance of TOP1 ribonucleoprotein complexes.

Main Content

This item is under embargo until July 7, 2022.