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TRF2, but not TBP, mediates the transcription of ribosomal protein genes.

  • Author(s): Wang, Yuan-Liang;
  • Duttke, Sascha HC;
  • Chen, Kai;
  • Johnston, Jeff;
  • Kassavetis, George A;
  • Zeitlinger, Julia;
  • Kadonaga, James T
  • et al.
Abstract

The TCT core promoter element is present in most ribosomal protein (RP) genes in Drosophila and humans. Here we show that TBP (TATA box-binding protein)-related factor TRF2, but not TBP, is required for transcription of the TCT-dependent RP genes. In cells, TCT-dependent transcription, but not TATA-dependent transcription, increases or decreases upon overexpression or depletion of TRF2. In vitro, purified TRF2 activates TCT but not TATA promoters. ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep sequencing) experiments revealed the preferential localization of TRF2 at TCT versus TATA promoters. Hence, a specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems.

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