Isolation and characterization of 9 polymorphic microsatellite markers for the endangered boring giant clam (Tridacna crocea) and cross-priming testing in three other Tridacnid species
- Author(s): DeBoer, Timery S.
- Barber, Paul H.
- et al.
Published Web Location
https://doi.org/10.1007/s12686-010-9249-7Abstract
We report the development and characterization of nine microsatellite markers from the Tridacna crocea genome. In a wild population of 20 individuals from Ulugan Bay in the Republic of the Philippines, we observed an average of 9 alleles per locus and expected heterozygosity ranging from 0.41 to 0.92. Cross-species priming was tested in three additional Tridacna species with moderate success. These markers will be used in studies of fine-scale population genetic structure and gene flow in T. crocea. The markers may also be useful in a forensic context by identifying source populations of giant clams collected illegally for the live aquarium trade, while protecting legal exports from aquaculture facilities.
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