HIV Vpr Engages the Host DDR and Chromatin-Associated Proteome
Engagement of the DDR by viruses in a broadly conserved mechanism among viruses and understanding how viruses modulate the DDR has paved the way to understanding several basic biological processes in molecular biology. Despite this, the role of engaging and modulating the DDR in the context of HIV remains poorly understood. For many years it has been apparent that the viral accessory gene Vpr plays a role in engaging the DDR during the viral lifecycle, however, the molecular processes and phenotypes associated with modulation remain poorly understood. For my dissertation research, we examine the function of HIV Vpr in the context of the DDR. First, we explore how Vpr engages and modulates the DDR by determining distinct cellular phenotypes specific to Vpr function to generate a model. We next explore the chromatin-associated proteome of Vpr to understand the underlying molecular mechanisms governing modulation of the DDR. Altogether, this will broaden our understanding HIV molecular biology in the context of the DDR and at chromatin.