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Aneuploid yeast strains exhibit defects in cell growth and passage through START.

  • Author(s): Thorburn, Rebecca R
  • Gonzalez, Christian
  • Brar, Gloria A
  • Christen, Stefan
  • Carlile, Thomas M
  • Ingolia, Nicholas T
  • Sauer, Uwe
  • Weissman, Jonathan S
  • Amon, Angelika
  • et al.
Abstract

Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate, and cell cycle events. Here we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as with many other cellular stresses, entry into the cell cycle.

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