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Genetic variation and temperature affects hybrid barriers during interspecific hybridization.

  • Author(s): Bjerkan, Katrine N
  • Hornslien, Karina S
  • Johannessen, Ida M
  • Krabberød, Anders K
  • van Ekelenburg, Yuri S
  • Kalantarian, Maryam
  • Shirzadi, Reza
  • Comai, Luca
  • Brysting, Anne K
  • Bramsiepe, Jonathan
  • Grini, Paul E
  • et al.

Published Web Location

https://doi.org/10.1111/tpj.14523Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Public License
Abstract

Genomic imprinting regulates parent-specific transcript dosage during seed development and is mainly confined to the endosperm. Elucidation of the function of many imprinted genes has been hampered by the lack of corresponding mutant phenotypes, and the role of imprinting is mainly associated with genome dosage regulation or allocation of resources. Disruption of imprinted genes has also been suggested to mediate endosperm based post-zygotic hybrid barriers depending on genetic variation and gene dosage. Here, we have analyzed the conservation of a clade from the MADS-box type I class transcription factors in the closely related species Arabidopsis arenosa, A. lyrata and A. thaliana, and show that AGL36-like genes are imprinted and maternally expressed in seeds of Arabidopsis species and in hybrid seeds between outbreeding species. In hybridizations between outbreeding and inbreeding species the paternally silenced allele of the AGL36-like gene is reactivated in the hybrid, demonstrating that also maternally expressed imprinted genes are perturbed during hybridization and that such effects on imprinted genes are specific to the species combination. Furthermore, we also demonstrate a quantitative effect of genetic diversity and temperature on the strength of the post-zygotic hybridization barrier. Remarkably, a small decrease in temperature during seed development increases survival of hybrid F1 seeds, suggesting that abiotic and genetic parameters play important roles in post-zygotic species barriers, pointing at evolutionary scenarios favoring such effects. This article is protected by copyright. All rights reserved.

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