Skip to main content
eScholarship
Open Access Publications from the University of California

High throughput sequencing of in vitro selections of mRNA-displayed peptides: data analysis and applications.

  • Author(s): Blanco, Celia
  • Verbanic, Samuel
  • Seelig, Burckhard
  • Chen, Irene A
  • et al.

Published Web Location

https://pubs.rsc.org/en/Content/ArticleLanding/2020/CP/C9CP05912A#!divAbstract
No data is associated with this publication.
Abstract

In vitro selection using mRNA display is currently a widely used method to isolate functional peptides with desired properties. The analysis of high throughput sequencing (HTS) data from in vitro evolution experiments has proven to be a powerful technique but only recently has it been applied to mRNA display selections. In this Perspective, we introduce aspects of mRNA display and HTS that may be of interest to physical chemists. We highlight the potential of HTS to analyze in vitro selections of peptides and review recent advances in the application of HTS analysis to mRNA display experiments. We discuss some possible issues involved with HTS analysis and summarize some strategies to alleviate them. Finally, the potential for future impact of advancing HTS analysis on mRNA display experiments is discussed.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Item not freely available? Link broken?
Report a problem accessing this item