Skip to main content
eScholarship
Open Access Publications from the University of California

A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae

  • Author(s): Piazza, A
  • Koszul, R
  • Heyer, WD
  • et al.
Abstract

© 2018 Elsevier Inc. Homologous recombination faithfully restores the sequence information interrupted by a DNA double-strand break by referencing an intact DNA molecule as a template for repair DNA synthesis. DNA synthesis is primed from 3′-OH end of the invading DNA strand in the displacement loop (D-loop). Here, we describe a simple and quantitative proximity ligation-based assay to study the initiation of homologous recombination-associated DNA synthesis initiated at the D-loop and final product formation. The D-loop extension assay overcomes the semiquantitative nature and some limitations of the current PCR-based technique and facilitates the study of the recombination-associated DNA synthesis.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Main Content
Current View