Relation between fluorescence spectra of dilute and turbid samples
- Author(s): Durkin, AJ
- Jaikumar, S
- Ramanujam, N
- Richards-Kortum, R
- et al.
Published Web Locationhttps://doi.org/10.1364/AO.33.000414
We present a method to extend rank-annihilation-factor analysis (RAFA) for the analysis of fluorescence from homogeneous turbid samples. The method is based on a fundamental relationship between the fluorescence of a dilute solution and that of a turbid solution. We have derived this relationship, known as the transfer function, for turbid materials using the two-flux Kubelka-Munk theory. The method is tested with spectroscopic data from optically thin and turbid samples of the media of a human aorta. At 450-nm excitation, agreement between the measured and predicted dilute-solution fluorescence spectra is within 5% at all emission wavelengths; at 340-nm excitation, agreement is within 20% at all wavelengths, with some residual Soret-band absorption. The simulations presented indicate that the transfer function is markedly more sensitive to absorption than to scattering properties. © 1994 Optical Society of America.
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