Skip to main content
Open Access Publications from the University of California

UC Irvine

UC Irvine Previously Published Works bannerUC Irvine

Human lymphotoxins: Purification to electrophoretic homogeneity of the αH receptor-bearing class


The au component (120-150,000 d) of the human LT system has been purified to electrophoretic homogeneity. This form was obtained from Con A-stimulated human tonsil and adenoid lymphocytes. Purification was greatly facilitated by employing a low concentration of lactalbumin hydrolysate as a serum substitute. The scheme consisted of sequential molecular sieving, lectin-affinity chromatography on Con A-Sepharose, and isoelectricfocusing. The specific activity of the αH increased approximately 5-10,000-fold in the course of purification, as judged by protein monitoring by the fluorescamine assay and radioiodination using the Iodogen technique. Homogeneity of the αH preparation from electrofocusing was demonstrated by native PAGE, as only a single protein peak was observed. The identity of this radioative peak with the lytic moiety was apparent as it migrated to the only region of the gel where lytic activity was detectable. This represents the third human lymphokine purified to homogeneity. © 1981.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View