UC Irvine

Knowledge of cerebral oxygenation is critical to an understanding of brain status in normal physiology and especially in development of pathophysiological conditions, such as cerebral amyloid angiopathy. This thesis describes development and evaluation of a simple multispectral reflectance imaging device. The device was modeled after a previously published paper by Dunn et al., and consists of a 3-D printed filter wheel, a CCD camera, a motor driving circuit, a photodiode, an Arduino, a light source and six bandpass filters. Two approaches to calculate differential pathlength factor (DPF) values were investigated and compared with published values from Dunn et al., and a surprising finding was that a DPF algorithm derived originally for point spectroscopy was the most accurate approach of the three to calculate changes in oxy- and deoxyhemoglobin. Further studies are warranted.