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Engineering prokaryotic transcriptional activators as metabolite biosensors in yeast
- Skjoedt, Mette L;
- Snoek, Tim;
- Kildegaard, Kanchana R;
- Arsovska, Dushica;
- Eichenberger, Michael;
- Goedecke, Tobias J;
- Rajkumar, Arun S;
- Zhang, Jie;
- Kristensen, Mette;
- Lehka, Beata J;
- Siedler, Solvej;
- Borodina, Irina;
- Jensen, Michael K;
- Keasling, Jay D
Published Web Location
https://doi.org/10.1038/nchembio.2177Abstract
Transplantation of the prokaryotic LysR-type transcriptional regulator into yeast combined with in vivo screening identifies yeast mutants that produce metabolic products with bacterial small molecule inducers.
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