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Identification of Adenosine-to-Inosine RNA Editing with Acrylonitrile Reagents
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https://doi.org/10.1021/acs.orglett.9b02929Abstract
New chemical probes have been designed to facilitate the identification of adenosine-to-inosine (A-to-I) edited RNAs. These reagents combine a conjugate acceptor for selective inosine covalent modification with functional groups for bioorthogonal biotinylation. The resulting biotinylated RNA was enriched and verified with RT-qPCR. This powerful chemical approach provides new opportunities to identify and quantify A-to-I editing sites.
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