Lawrence Berkeley National Laboratory
All-in-one nanowire-decorated multifunctional membrane for rapid cell lysis and direct DNA isolation.
- Author(s): So, Hongyun
- Lee, Kunwoo
- Murthy, Niren
- Pisano, Albert
- et al.
Published Web Locationhttps://doi.org/10.1021/am506153y
This paper describes a handheld device that uses an all-in-one membrane for continuous mechanical cell lysis and rapid DNA isolation without the assistance of power sources, lysis reagents, and routine centrifugation. This nanowire-decorated multifunctional membrane was fabricated to isolate DNA by selective adsorption to silica surface immediately after disruption of nucleus membranes by ultrasharp tips of nanowires for a rapid cell lysis, and it can be directly assembled with commercial syringe filter holders. The membrane was fabricated by photoelectrochemical etching to create microchannel arrays followed by hydrothermal synthesis of nanowires and deposition of silica. The proposed membrane successfully purifies high-quality DNA within 5 min, whereas a commercial purification kit needs more than an hour.