UC San Diego

Interferons (IFNs) are a family of cytokines that play important roles in the innate immune system. They exhibit antiviral effects through the induction of Interferon Stimulated Genes (ISGs), which includes the Schlafen genes. Here, we reported human Schlafen 5 (huSlfn5) localized mainly to the cytoplasm in human foreskin fibroblasts (HFF) and mainly to the nucleus in Hela cells. Treatment with human interferon [Beta] and co-transfection with pNL4-3.Luc.R⁺E⁻ HIV-1 vectors did not cause significant changes in the localization pattern of huSlfn5. In addition, we found human Schlafen11 (huSlfn11) to localize exclusively to the cytoplasm in HFF cells but exclusively to the nucleus in Hela cells. This indicates that the subcellular localization of human Slfn11 proteins are cell-type specific. HuIFN[Beta] stimulation and pNL4- 3.Luc.R⁺E⁻ HIV-1 vector co-transfection did not have any prominent effect on the localization of the huSfln11 proteins