Nucleotide sequence and transcription of a trypomastigote surface antigen gene of Trypanosoma cruzi
- Author(s): Fouts, DL
- Ruef, BJ
- Ridley, PT
- Wrightsman, RA
- Peterson, DS
- Manning, JE
- et al.
Published Web Locationhttps://doi.org/10.1016/0166-6851(91)90043-6
In previous studies we identified a 500-bp segment of the gene, TSA-1, which encodes an 85-kDa trypomastigote-specific surface antigen of the Peru strain of Trypanosoma cruzi. TSA-1 was shown to be located at a telomeric site and to contain a 27-bp tandem repeat unit within the coding region. This repeat unit defines a discrete subset of a multigene family and places the TSA-1 gene within this subset. In this study, we present the complete nucleotide sequence of the TSA-1 gene from the Peru strain. By homology matrix analysis, fragments of two other trypomastigote specific surface antigen genes, pTt34 and SA85-1.1, are shown to have extensive sequence homology with TSA-1 indicating that these genes are members of the same gene family as TSA-1. The TSA-1 subfamily was also found to be active in two other strains of T. cruzi, one of which contains multiple telomeric members and one of which contains a single non-telomeric member, suggesting that transcription is not necessarily dependent on the gene being located at a telomeric site. Also, while some of the sequences found in this gene family are present in 2 size classes of poly(A)+RNA, others appear to be restricted to only 1 of the 2 RNA classes. © 1991.
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