Synthesis of Tag-Pollutants Conjugates for Synthetically Evolved Receptors Screening
- Author(s): Huang, Jianbo
- Advisor(s): Siegel, Dionicio
- et al.
Fast on-site detection of organic pollutants using biosensors is developing rapidly. To select Nanobodies which have binding specificity to varies of pollutants to make the biosensors, the pollutants and tag conjugates was synthesized in lab and applied to cell culture. Our results demonstrated that the recombination of the naïve nanobody culture for generations does produces the nanobodies possessing high binding affinity towards organic pollutants.