Frequency-domain phase-modulation fluorometry: Resolution of complex decays of fluorescence intensity and anisotropy
- Author(s): Lakowicz, JR
- Cherek, H
- Maliwal, B
- Gratton, E
- et al.
Published Web Locationhttps://doi.org/10.1016/0022-2313(84)90100-5
We used frequency-domain fluorometry to resolve multi-exponential decays of fluorescence intensity and anisotropy. The samples are excited with intensity modulated light, at modulation frequencies ranging from 1 to 200 MHz, and we measure the phase-shifts and demodulation factors. The decay times of a three-component mixture were resolved, 1.2, 4.1 and 7.7 nsec, as well as the two anisotropy decay times of the anisotropic rotator perylene. © 1984.