Skip to main content
Open Access Publications from the University of California

UC Irvine

UC Irvine Previously Published Works bannerUC Irvine

Frequency-domain phase-modulation fluorometry: Resolution of complex decays of fluorescence intensity and anisotropy


We used frequency-domain fluorometry to resolve multi-exponential decays of fluorescence intensity and anisotropy. The samples are excited with intensity modulated light, at modulation frequencies ranging from 1 to 200 MHz, and we measure the phase-shifts and demodulation factors. The decay times of a three-component mixture were resolved, 1.2, 4.1 and 7.7 nsec, as well as the two anisotropy decay times of the anisotropic rotator perylene. © 1984.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View