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Open Access Publications from the University of California

Frequency-domain phase-modulation fluorometry: Resolution of complex decays of fluorescence intensity and anisotropy

  • Author(s): Lakowicz, JR
  • Cherek, H
  • Maliwal, B
  • Gratton, E
  • et al.

We used frequency-domain fluorometry to resolve multi-exponential decays of fluorescence intensity and anisotropy. The samples are excited with intensity modulated light, at modulation frequencies ranging from 1 to 200 MHz, and we measure the phase-shifts and demodulation factors. The decay times of a three-component mixture were resolved, 1.2, 4.1 and 7.7 nsec, as well as the two anisotropy decay times of the anisotropic rotator perylene. © 1984.

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