Skip to main content
eScholarship
Open Access Publications from the University of California

UCLA

UCLA Previously Published Works bannerUCLA

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.

  • Author(s): Donnelly, Daniel P;
  • Rawlins, Catherine M;
  • DeHart, Caroline J;
  • Fornelli, Luca;
  • Schachner, Luis F;
  • Lin, Ziqing;
  • Lippens, Jennifer L;
  • Aluri, Krishna C;
  • Sarin, Richa;
  • Chen, Bifan;
  • Lantz, Carter;
  • Jung, Wonhyeuk;
  • Johnson, Kendall R;
  • Koller, Antonius;
  • Wolff, Jeremy J;
  • Campuzano, Iain DG;
  • Auclair, Jared R;
  • Ivanov, Alexander R;
  • Whitelegge, Julian P;
  • Paša-Tolić, Ljiljana;
  • Chamot-Rooke, Julia;
  • Danis, Paul O;
  • Smith, Lloyd M;
  • Tsybin, Yury O;
  • Loo, Joseph A;
  • Ge, Ying;
  • Kelleher, Neil L;
  • Agar, Jeffrey N
  • et al.
Abstract

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View