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FRET-based trilateration of probes bound within functional ryanodine receptors

  • Author(s): Svensson, B
  • Oda, T
  • Nitu, FR
  • Yang, Y
  • Cornea, I
  • Chen-Izu, Y
  • Fessenden, JD
  • Bers, DM
  • Thomas, DD
  • Cornea, RL
  • et al.

Published Web Location

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223179/
No data is associated with this publication.
Abstract

© 2014 Biophysical Society. To locate the biosensor peptide DPc10 bound to ryanodine receptor (RyR) Ca2+channels, we developed an approach that combines fluorescence resonance energy transfer (FRET), simulated-annealing, cryo-electron microscopy, and crystallographic data. DPc10 is identical to the 2460-2495 segment within the cardiac muscle RyR isoform (RyR2) central domain. DPc10 binding to RyR2 results in a pathologically elevated Ca2+leak by destabilizing key interactions between the RyR2 N-terminal and central domains (unzipping). To localize the DPc10 binding site within RyR2, we measured FRET between five single-cysteine variants of the FK506-binding protein (FKBP) labeled with a donor probe, and DPc10 labeled with an acceptor probe (A-DPc10). Effective donor positions were calculated from simulated-annealing constrained by both the RyR cryo-EM map and the FKBP atomic structure docked to the RyR. FRET to A-DPc10 was measured in permeabilized cardiomyocytes via confocal microscopy, converted to distances, and used to trilaterate the acceptor locus within RyR. Additional FRET measurements between donor-labeled calmodulin and A-DPc10 were used to constrain the trilaterations. Results locate the DPc10 probe within RyR domain 3, ∼35 Å from the previously docked N-terminal domain crystal structure. This multiscale approach may be useful in mapping other RyR sites of mechanistic interest within FRET range of FKBP.

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