Lawrence Berkeley National Laboratory
A cephalosporin-chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude.
- Author(s): Maity, Santanu
- Wang, Xiaojian
- Das, Subhamoy
- He, Maomao
- Riley, Lee W
- Murthy, Niren
- et al.
Published Web Locationhttps://pubs.rsc.org/en/content/articlelanding/2020/CC/C9CC09498A#!divAbstract
The expression of beta-lactamases in bacteria is a central cause of drug resistance. In this report, we present a beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and can detect beta lactamase with a sensitivity that is 4-orders of magnitude higher than the commercially available fluorescent lactamase substrate fluorocillin.