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Optimization of the 454 Production Sequencing Workflow at the DOE Joint Genome Institute

  • Author(s): Daum, Christopher
  • Hamilton, Matthew
  • Shapiro, Nicole
  • Robinson, David
  • Hack, Christopher
  • Kennedy, Megan
  • Pollard, Marty
  • Roberts, Simon
  • Humphries, David
  • Cheng, Jan-Fang
  • Lucas, Susan
  • et al.
Abstract

Within the Production Sequencing group a robust Roche GS-FLX sequencer pipeline has been established. Optimization of the pipeline has been ongoing with the aim of continual process improvement of the laboratory workflow. These process improvement projects are being lead by the JGIs Process Optimization, Sequencing Technologies, Instrumentation and Engineering, and the core 454 Production groups. Primary focus has been on improving the procedural ergonomics and the technicians operating environment, reducing manually intensive technician operations with tools and robotic automation, reducing associated production costs, and improving the overall process and generated sequence quality.

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