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Caudal counter-represses Hunchback to regulate even-skipped stripe 2 expression in Drosophila embryos

  • Author(s): Vincent, Ben
  • Staller, Max
  • Lopez-Rivera, Francheska
  • Bragdon, Meghan DJ
  • Wunderlich, Zeba
  • Estrada, Javier
  • DePace, Angela
  • et al.

Published Web Location

https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007644
No data is associated with this publication.
Abstract

Hunchback is a bifunctional transcription factor that can activate and repress gene expression in Drosophila development. We investigated the regulatory DNA sequence features that control Hunchback function by perturbing enhancers for one of its target genes, even-skipped . While Hunchback directly represses the eve stripe 3+7 enhancer, we found that in the eve stripe 2+7 enhancer, Hunchback repression is prevented by Caudal binding—this relationship is called counter-repression. We found evidence that this relationship is conserved by comparing predicted binding sites for Hunchback and Caudal across orthologous eve stripe 2 enhancers. These results alter the textbook view of eve stripe 2 regulation wherein Hb is depicted as a direct activator. Instead, to generate stripe 2, Hunchback repression must be counteracted by Caudal binding. We discuss the implications of this interaction for eve stripe 2 regulation and evolution.

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