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Factors that Influence the Formation and Stability of Thin, Cryo-EM Specimens.

  • Author(s): Glaeser, Robert M
  • Han, Bong-Gyoon
  • Csencsits, Roseann
  • Killilea, Alison
  • Pulk, Arto
  • Cate, Jamie HD
  • et al.

Published Web Location

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4775786/
No data is associated with this publication.
Abstract

Poor consistency of the ice thickness from one area of a cryo-electron microscope (cryo-EM) specimen grid to another, from one grid to the next, and from one type of specimen to another, motivates a reconsideration of how to best prepare suitably thin specimens. Here we first review the three related topics of wetting, thinning, and stability against dewetting of aqueous films spread over a hydrophilic substrate. We then suggest that the importance of there being a surfactant monolayer at the air-water interface of thin, cryo-EM specimens has been largely underappreciated. In fact, a surfactant layer (of uncontrolled composition and surface pressure) can hardly be avoided during standard cryo-EM specimen preparation. We thus suggest that better control over the composition and properties of the surfactant layer may result in more reliable production of cryo-EM specimens with the desired thickness.

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