Preparation of splicing competent nuclear extracts
Published Web Locationhttps://doi.org/10.1007/978-1-62703-980-2_8
Splicing components play an essential role in mediating accurate and efficient splicing. The complexity of the spliceosome and its regulatory networks increase the difficulty of studying the splicing reaction in detail. Nuclear extracts derived from HeLa cells provide all of the obligatory components to carry out intron removal in vitro. This chapter describes the large-scale preparation of nuclear extract from HeLa cells. © 2014 Springer Science+Business Media, LLC.