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Open Access Publications from the University of California

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.

  • Author(s): Wu, Jianglai
  • Liang, Yajie
  • Chen, Shuo
  • Hsu, Ching-Lung
  • Chavarha, Mariya
  • Evans, Stephen W
  • Shi, Dongqing
  • Lin, Michael Z
  • Tsia, Kevin K
  • Ji, Na
  • et al.

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.

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