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Synthesis and bioluminescence of thioluciferin

Published Web Location

https://doi.org/10.1016/j.bmcl.2019.07.050
Abstract

The firefly luciferin analog thioluciferin (S-luc) was synthesised as a key element of bioluminescent reporters for oxidation state and thiol/disulfide equilibria. It shows blue-shifts in absorption and fluorescence compared to luciferin, and is a modest luciferase substrate. These features are attributed to a π-system that is less conjugated than luciferin.

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