Skip to main content
eScholarship
Open Access Publications from the University of California
Refine Search

UC Berkeley

UC Berkeley Previously Published Works bannerUC Berkeley

Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors.

Published Web Location

https://doi.org/10.1038/nmeth.4483
Abstract

Targeted and inducible regulation of mammalian gene expression is a broadly important capability. We engineered drug-inducible catalytically inactive Cpf1 nuclease fused to transcriptional activation domains to tune the expression of endogenous genes in human cells. Leveraging the multiplex capability of the Cpf1 platform, we demonstrate both synergistic and combinatorial gene expression in human cells. Our work should enable the development of multiplex gene perturbation library screens for understanding complex cellular phenotypes.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
For improved accessibility of PDF content, download the file to your device.
Current View


Top