UC Santa Cruz

In this work, we combined the micropore fabrication process into the fabrication processes of the microfluidic device. With certain post-treatment processes, we integrated solid-state nanopore with the microfluidic chip and created a nanopore-microfluidic platform. On this platform, we demonstrated the first detection of NaCMC (Sodium Carboxymethyl Cellulose) with a nanopore. Based on the demonstration of nucleic acids and NaCMC detection separately on this platform, we successfully demonstrated the multiplex detection in the mixture of NaCMC and λ-DNA. With the combination of feedback controlled gated system, we also demonstrated a controlled number of NaCMC delivery on this platform. In order to improve the solid-state nanopore sensitivity in the nanopore-microfluidic platform, we successfully performed ion-sculpting on the direct drilling nanopores. Electrical properties of the ion-sculpted nanopore have been investigated preliminarily, and NaCMC detection on the ion-sculpted nanopore has been demonstrated.