Skip to main content
Open Access Publications from the University of California

Ratiometric Imaging of pH Probes

  • Author(s): Grillo-Hill, BK
  • Webb, BA
  • Barber, DL
  • et al.

Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis. © 2014 Elsevier Inc.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View