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Ratiometric Imaging of pH Probes

  • Author(s): Grillo-Hill, BK
  • Webb, BA
  • Barber, DL
  • et al.
Abstract

Measurement of intracellular pH can be readily accomplished using tools and methods described in this chapter. We present a discussion of technical considerations of various ratiometric pH-sensitive probes including dyes and genetically encoded sensors. These probes can be used to measure pH across physical scales from macroscopic whole-mount tissues down to organelles and subcellular domains. We describe protocols for loading pH-sensitive probes into single cells or tissues and discuss ratiometric image acquisition and analysis. © 2014 Elsevier Inc.

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