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High-Throughput Screening of Encapsulated Islets Using Wide-Field Lens-Free On-Chip Imaging

  • Author(s): Zhang, Y
  • Alexander, M
  • Yang, S
  • Bian, Y
  • Botvinick, E
  • Lakey, JRT
  • Ozcan, A
  • et al.
Abstract

Islet microencapsulation is a promising solution to diabetes treatment, but its quality control based on manual microscopic inspection is extremely low-throughput, highly variable, and laborious. This study presents a high-throughput islet-encapsulation quality screening system based on lens-free on-chip imaging with a wide field-of-view of 18.15 cm , which is more than 100× larger than that of a lens-based optical microscope, enabling it to image and analyze ∼8000 microcapsules in a single frame. Custom-written image reconstruction and processing software provides the user with clinically important information, such as microcapsule count, size, intactness, and information on whether each capsule contains an islet. This high-throughput and cost-effective platform can be useful for researchers to develop better encapsulation protocols as well as perform quality control prior to transplantation. 2

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