High-Throughput Screening of Encapsulated Islets Using Wide-Field Lens-Free On-Chip Imaging
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High-Throughput Screening of Encapsulated Islets Using Wide-Field Lens-Free On-Chip Imaging

  • Author(s): Zhang, Yibo
  • Alexander, Michael
  • Yang, Sam
  • Bian, Yinxu
  • Botvinick, Elliot
  • Lakey, Jonathan RT
  • Ozcan, Aydogan
  • et al.
Abstract

Islet microencapsulation is a promising solution to diabetes treatment, but its quality control based on manual microscopic inspection is extremely low-throughput, highly variable and laborious. This study presents a high-throughput islet-encapsulation quality screening system based on lens-free on-chip imaging with a wide field-of-view of 18.15 cm^2, which is more than 100 times larger than that of a lens-based optical microscope, enabling it to image and analyze ~8,000 microcapsules in a single frame. Custom-written image reconstruction and processing software provides the user with clinically important information, such as microcapsule count, size, intactness, and information on whether each capsule contains an islet. This high-throughput and cost-effective platform can be useful for researchers to develop better encapsulation protocols as well as perform quality control prior to transplantation.

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