Covalent, sequence-specific attachment of long DNA molecules to a surface using DNA-templated click chemistry.
- Author(s): Abel, Gary R
- Cao, Blessing Huynh
- Hein, Jason E
- Ye, Tao
- et al.
Published Web Locationhttps://doi.org/10.1039/c4cc02900c
We present a novel method that covalently and sequence-specifically attaches long DNA molecules to a surface that is compatible with high-resolution atomic force microscopy (AFM) imaging. Surfaces prepared with this approach are ideally suited for performing biophysical experiments on single DNA molecules.