Skip to main content
eScholarship
Open Access Publications from the University of California

T cell antigen discovery via signaling and antigen-presenting bifunctional receptors.

  • Author(s): Joglekar, Alok V
  • Leonard, Michael T
  • Jeppson, John D
  • Swift, Margaret
  • Li, Guideng
  • Wong, Stephanie
  • Peng, Songming
  • Zaretsky, Jesse M
  • Heath, James R
  • Ribas, Antoni
  • Bethune, Michael T
  • Baltimore, David
  • et al.
Abstract

CD8+ T cells recognize and eliminate tumors in an antigen-specific manner. Despite progress in characterizing the antitumor T cell repertoire and function, the identification of target antigens remains a challenge. Here we describe the use of chimeric receptors called signaling and antigen-presenting bifunctional receptors (SABRs) in a cell-based platform for T cell receptor (TCR) antigen discovery. SABRs present an extracellular complex comprising a peptide and major histocompatibility complex (MHC), and induce intracellular signaling via a TCR-like signal after binding with a cognate TCR. We devised a strategy for antigen discovery using SABR libraries to screen thousands of antigenic epitopes. We validated this platform by identifying the targets recognized by public TCRs of known specificities. Moreover, we extended this approach for personalized neoantigen discovery.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View