A multi-model approach to assessing local and global cryo-EM map quality
- Author(s): Herzik, Mark
- Fraser, James
- Lander, Gabriel
- et al.
Published Web Locationhttps://www.biorxiv.org/content/biorxiv/early/2017/04/19/128561.full.pdf
Abstract There does not currently exist a standardized indicator of how well a cryo-EM-derived model represents the density from which it was generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected model–model agreement criterion for EM maps reported to be at 5 Å resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality.