Live flow cytometry analysis of c-di-GMP levels in single cell populations
- Author(s): Yeo, J
- Wang, XC
- Hammond, MC
- et al.
Published Web Locationhttps://www.ncbi.nlm.nih.gov/pubmed/?term=Live+flow+cytometry+analysis+of+c-di-GMP+levels+in+single+cell+populations
© Springer Science+Business Media LLC 2017. Second-generation RNA-based fluorescent biosensors have been developed that enable flow cytometry experiments to monitor the population dynamics of c-di-GMP signaling in live bacteria. These experiments are high-throughput, provide information at the single-cell level, and can be performed on cells grown in complex media and/or under anaerobic conditions. Here, we describe flow cytometry methods for three applications: (1) high-throughput screening for diguanylate cyclase activity, (2) analyzing c-di-GMP levels under anaerobic conditions, and (3) monitoring cell population dynamics of c-di-GMP levels upon environmental changes. These methods showcase RNA-based fluorescent biosensors as versatile tools for studying c-di-GMP signaling in bacteria.
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This item is under embargo until July 26, 2019.
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