Lawrence Berkeley National Laboratory
EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
- Author(s): Barad, Benjamin A
- Echols, Nathaniel
- Wang, Ray Yu-Ruei
- Cheng, Yifan
- DiMaio, Frank
- Adams, Paul D
- Fraser, James S
- et al.
Published Web Locationhttps://doi.org/10.1038/nmeth.3541
Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.