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EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.

  • Author(s): Barad, Benjamin A;
  • Echols, Nathaniel;
  • Wang, Ray Yu-Ruei;
  • Cheng, Yifan;
  • DiMaio, Frank;
  • Adams, Paul D;
  • Fraser, James S
  • et al.
Abstract

Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.

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