Skip to main content
eScholarship
Open Access Publications from the University of California

A rapid method for determining protein diffusion through hydrogels for regenerative medicine applications.

  • Author(s): Hettiaratchi, Marian H
  • Schudel, Alex
  • Rouse, Tel
  • García, Andrés J
  • Thomas, Susan N
  • Guldberg, Robert E
  • McDevitt, Todd C
  • et al.

Published Web Location

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324205/
No data is associated with this publication.
Abstract

Hydrogels present versatile platforms for the encapsulation and delivery of proteins and cells for regenerative medicine applications. However, differences in hydrogel cross-linking density, polymer weight content, and affinity for proteins all contribute to diverse diffusion rates of proteins through hydrogel networks. Here, we describe a simple method to accurately measure protein diffusion through hydrogels, within a few hours and without the use of large amounts of protein. We tracked the diffusion of several proteins of varying molecular weights along the axial direction of capillary tubes filled with alginate, collagen, or poly(ethylene glycol) hydrogels. The rate of protein diffusion decreased with increasing molecular weight. A computational model of protein diffusion through capillary tubes was also created to predict and verify experimental protein diffusion coefficients. This in vitro capillary tube-based method of measuring protein diffusion represents a simple strategy to interrogate protein diffusion through natural and synthetic hydrogels and aid in the design of better biomaterial-based delivery vehicles that can effectively modulate protein release.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Item not freely available? Link broken?
Report a problem accessing this item