Skip to main content
eScholarship
Open Access Publications from the University of California

In vivo targeting of de novo DNA methylation by histone modifications in yeast and mouse.

  • Author(s): Morselli, Marco
  • Pastor, William A
  • Montanini, Barbara
  • Nee, Kevin
  • Ferrari, Roberto
  • Fu, Kai
  • Bonora, Giancarlo
  • Rubbi, Liudmilla
  • Clark, Amander T
  • Ottonello, Simone
  • Jacobsen, Steven E
  • Pellegrini, Matteo
  • et al.
Abstract

Methylation of cytosines (5(me)C) is a widespread heritable DNA modification. During mammalian development, two global demethylation events are followed by waves of de novo DNA methylation. In vivo mechanisms of DNA methylation establishment are largely uncharacterized. Here, we use Saccharomyces cerevisiae as a system lacking DNA methylation to define the chromatin features influencing the activity of the murine DNMT3B. Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this observation, DNA methylation analysis in yeast strains without Set1 and Set2 shows an increase of relative 5(me)C levels at the transcription start site and a decrease in the gene-body, respectively. We extend our observation to the murine male germline, where H3K4me3 is strongly anti-correlated while H3K36me3 correlates with accelerated DNA methylation. These results show the importance of H3K36 methylation for gene-body DNA methylation in vivo.

Many UC-authored scholarly publications are freely available on this site because of the UC's open access policies. Let us know how this access is important for you.

Main Content
Current View