Skip to main content
Open Access Publications from the University of California

CasX enzymes comprise a distinct family of RNA-guided genome editors.

  • Author(s): Liu, Jun-Jie
  • Orlova, Natalia
  • Oakes, Benjamin L
  • Ma, Enbo
  • Spinner, Hannah B
  • Baney, Katherine LM
  • Chuck, Jonathan
  • Tan, Dan
  • Knott, Gavin J
  • Harrington, Lucas B
  • Al-Shayeb, Basem
  • Wagner, Alexander
  • Brötzmann, Julian
  • Staahl, Brett T
  • Taylor, Kian L
  • Desmarais, John
  • Nogales, Eva
  • Doudna, Jennifer A
  • et al.

Published Web Location
No data is associated with this publication.

The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.

Many UC-authored scholarly publications are freely available on this site because of the UC Academic Senate's Open Access Policy. Let us know how this access is important for you.

Item not freely available? Link broken?
Report a problem accessing this item